• 3D reconstruction and animation of the hippocampus from a GFP-M mouse.

• Ultramicroscope creates high-resolution 3D-pictures of flies

• The simulation of a scan through entire cleared brain from a GFP-M mouse

UltraMicroscope II觀察內(nèi)源性熒光蛋白以實(shí)現(xiàn)體內(nèi)成像

A) 小鼠胚胎E12, 整體,三種標(biāo)記;
圖片由Serge van de Pavert,
VU Amsterdam提供
B) 果蠅幼蟲(chóng),自發(fā)熒光;
圖片由LaVision BioTec公司提供

UltraMicroscope II結(jié)合3DISCO技術(shù)用于研究小鼠神經(jīng)元軸突連接

UltraMicroscopeII用于觀察神經(jīng)膠質(zhì)瘤模型的抗體聚集

UltraMicroscope II用于研究小鼠胚胎初始淋巴管的形成機(jī)制

在哺乳動(dòng)物發(fā)育過(guò)程中，主靜脈血管中的內(nèi)皮細(xì)胞亞群表達(dá)淋巴管特異基因，進(jìn)而發(fā)育出初級(jí)的淋巴結(jié)構(gòu)-淋巴囊。淋巴內(nèi)皮細(xì)胞的產(chǎn)生經(jīng)過(guò)了出芽，擴(kuò)展和膨脹的過(guò)程，但是淋巴管形成的確切機(jī)制仍然不完全清楚。使用UltraMicroscope II來(lái)觀察整體免疫染色的小鼠胚胎，我們以細(xì)胞水平的分辨率觀察到了完整的發(fā)育中的血管系統(tǒng)。實(shí)驗(yàn)發(fā)現(xiàn)最早的淋巴內(nèi)皮細(xì)胞寬松地連接了主靜脈和淺靜脈叢，進(jìn)而聚集產(chǎn)生了兩個(gè)新發(fā)現(xiàn)的淋巴結(jié)構(gòu)，背部外周縱向淋巴管和腹側(cè)初級(jí)胸導(dǎo)管，最后與主靜脈相連。進(jìn)一步研究其功能，發(fā)現(xiàn)血管內(nèi)皮生長(zhǎng)因子C和基質(zhì)組分CCBE1對(duì)于淋巴內(nèi)皮細(xì)胞出芽和遷移是必需的。研究使用ltraMicroscope II詳細(xì)觀察了淋巴管的發(fā)育并構(gòu)建了新的模型。

UltraMicroscope II用于觀察人類胚胎早期生長(zhǎng)發(fā)育

UltraMicroscope II用于闡釋脂肪代謝神經(jīng)調(diào)控機(jī)制

部分應(yīng)用文獻(xiàn)

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